植物-微生物-昆虫互作研究组(黄伟杰)
研究组长:黄伟杰研究员
黄伟杰,中国科学院分子植物科学卓越创新中心研究员、课题组长、博士生导师。2008年本科毕业于华东师范大学,2015年博士毕业于中国科学院植物生理生态研究所,2015-2022年在英国约翰-英纳斯中心(John Innes Centre)从事博士后研究,2022年加入分子植物科学卓越创新中心。研究方向为植物-病原菌-昆虫互组,重点围绕虫媒细菌性植物病害开展研究,近年来在Cell,MolecularPlant,PlantCell等期刊发表多篇论文。
主要研究方向及内容:
虫媒植物病害,包括多数病毒病害、部分细菌和真菌病害,给农业生产和粮食安全带来严重危害。同时,虫媒病害的防治高度依赖于化学杀虫剂,也引发了环境污染和农药残留等诸多问题。近年来,以韧皮部细菌病害为代表的虫媒病害愈演愈烈,已成为全球农业生产的重大威胁之一。植原体作为一种典型的虫媒细菌性病害,由叶蝉、飞虱等刺吸式昆虫取食植物而传播,特异侵染植物的韧皮部组织,能够侵染上千种植物。实际上,植原体既侵染植物也侵染昆虫。更有意思的是,植原体常常会引起植物宿主发育上的剧烈变化,包括让植物的花变成叶子(phyllody)或者让植物产生小叶和丛枝(Witches' broom)等症状,使得植物无法正常生长繁殖,从而沦为植原体滋生和虫媒传播的温床,又被称为‘僵尸’植物(‘Zombie’plant)。因此,植原体病害为研究植物韧皮部免疫应答以及韧皮部-病原菌-昆虫相互作用提供了一个理想的模式系统。本课题组将围绕重大植原体病害,从生物互作角度出发,结合微生物学,昆虫学,植物发育学,植物免疫学,作物育种学等多项学科,在基础研究和应用研究两方面开展工作,力争为韧皮部病虫害的防治提供理论依据和新方法。主要研究方向包括:
1. 植原体如何调控植物宿主发育和植物-昆虫相互作用?
2. 植原体如何侵染昆虫以及影响昆虫宿主行为?
3. 植物韧皮部存在哪些免疫应答机制?
4. 如何改造植物使之对韧皮部病虫害产生抗性?
5. 植原体效应因子利用非泛素依赖蛋白降解途调控宿主发育的分子机制解析。
研究队伍:
科研助理:李亚璐
博士后: 刘言志
硕博研究生:王小艺,李媛媛,李田田,惠佳明
发表论文:
1.Weijie Huang, Allyson M. MacLean, Akiko Sugio, Abbas Maqbool, Marco Busscher, Shu-Ting Ch, Sophien Kamoun, Chih-Horng Kuo, Richard G.H. Immink, Saskia A. Hogenhout. (2021). Parasitic modulation of host development by ubiquitin-independent protein degradation. Cell 184: 5201-5214.
2.Weijie Huang*, Paola Reyes-Caldas*, Marina Mann*, Shirin Seifbarghi*, Alexandra Kahn*, Rodrigo P.P. Almeida, Laure Beven, Michelle Heck, Saskia A. Hogenhout, Gitta Coaker. (2020). Bacterial Vector-Borne Plant Diseases: Unanswered Questions and Future Directions. Molecular Plant 13: 1379-1393.
3.Wei-Jie Huang, Hai-Kuan Liu, Sheila McCormick, Wei-Hua Tang. (2014) Tomato Pistil Factor STIG1 Promotes in Vivo Pollen Tube Growth by Binding to Phosphatidylinositol 3-Phosphate and the Extracellular Domain of the Pollen Receptor Kinase LePRK2. Plant Cell 26: 2505-2523.
4.Ting-Lu Yuan*, Wei-Jie Huang*, Juan He, Dong Zhang, Wei-Hua Tang. (2018) Stage-Specific Gene Profiling of Germinal Cells Helps Delineate the Mitosis/Meiosis Transition. Plant Physiology 176: 1610-1626.
5.Wei-Jie Huang, Wei-Hua Tang. (2015). Measurement of Cellular Redox in Pollen with Redox-Sensitive GFP (roGFP) Using Live Cell Imaging. bio-Protocol 5.
6. Shu-Ting Cho, Hung-Jui Kung, Weijie Huang, Saskia A. Hogenhout, Chih-Horng Kuo. (2020). Species boundaries and molecular markers for classification among 16SrI phytoplasmas informed by genome analysis. Frontiers in Microbiology 11:1531.
7.Silke Lehmann, Ana Dominguez-Ferreras, Wei-Jie Huang, Katherine Denby, Vardis Ntoukakis, Patrick Sch?fer. (2020) Novel markers for high-throughput protoplast-based analyses of phytohormone signaling. PLoS One 15.
8.Shu-Ting Cho, Agnieszka Zwolińska, Weijie Huang, Roland H M Wouters, Sam T Mugford, Saskia A Hogenhout, Chih-Horng Kuo. (2020) Complete Genome Sequence of " Candidatus Phytoplasma asteris" RP1 66, a Plant Pathogen Associated with Rapeseed Phyllody Disease in Poland. Microbiology Resource Announcements 9.
9.Hai-Kuan Liu, Yu-Jie Li, Shu-Jie Wang, Ting-Lu Yuan, Wei-Jie Huang, Xin Dong, Jia-Qi Pei, Dong Zhang, Sheila McCormick, Wei-Hua Tang. (2020) Kinase Partner Protein Plays a Key Role in Controlling the Speed and Shape of Pollen Tube Growth in Tomato. Plant Physiology 184: 1853-1869,
10.Cai-Ping Gui, Xin Dong, Hai-Kuan Liu, Wei-Jie Huang; Dong Zhang, Shu-Jie Wang, María Laur a Barberini, Xiao-Yan Gao, Jorge Muschietti, Sheila McCormick, Wei-Hua Tang. (2014) Overexpression of the Tomato Pollen Receptor Kinase LePRK1 Rewires Pollen Tube Growth to a Blebbing Mode. Plant Cell 26: 3538-3555.
11.Justin Clements, Benjamin Z Bradford, Marjorie Garcia, Shannon Piper, Weijie Huang, Agnieszka Zwolinska, Kurt Lamour, Saskia Hogenhout, Russell L Groves. (2021). 'Candidatus Phytoplasma asteris' subgroups display distinct disease progression dynamics during the carrot growing season. PLoS One 16.
12.Morgan Lee, Anna Dominguez-Ferreras, Ewon Kaliyadasa, Wei-Jie Huang, Edna Antony, Tracey Stevenson, Silke Lehmann, Patrick Sch?fer, Marc R. Knight, Vardis Ntoukakis, Heather Knight. (2021) Mediator subunits MED16, MED14 and MED2 are required for activation of ABRE-dependent transcription in Arabidopsis. Frontiers in Plant Science 12.
13.María Laura Barberini, Lorena Sigaut, Weijie Huang, Silvina Mangano, Silvina Paola Denita Juarez, Eliana Marzol, José Estevez, Mariana Obertello, Lía Pietrasanta, Weihua Tang, Jorge Muschietti. (2017) Calcium dynamics in tomato pollen tubes using the Yellow Cameleon 3.6 sensor. Plant reproduction 21: 159-169.
14.Yan Bao, Wei-Meng Song, Yan-Li Jin, Chun-Mei Jiang, Yang Yang, Bei Li, Wei-Jie Huang, Hua Liu, Hong-Xia Zhang. Characterization of Arabidopsis Tubby-like proteins and redundant function of AtTLP3 and AtTLP9 in plant response to ABA and osmotic stress. Plant Molecular Biology 86: 471-483.