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National Key Laboratory of Plant Molecular Genetics
National Center of Plant Gene Research (Shanghai)
National Center for Gene Research, CAS
Key Laboratory of Synthetic Biology
Laboratory of Photosynthesis and Environmental Biology
Key Laboratory of Insect Developmental and Evolutionary Biology, CAS
Shanghai Entomological Museum, CAS
Key Laboratory of Synthetic Biology

The Key Laboratory of Synthetic Biology was established in Chinese Academy of Science in 2008 through reorganization of the Laboratory of Molecular Microbiology, The laboratory currently consists of 7 research groups including 12 principal investigators and about 70 graduate students. The director of laboratory is Prof. Guo-Ping Zhao and the academic committee director is Prof. Sheng-Li Yang.

The Laboratory aims at the cutting-edge research in synthetic biology to address global challenges in areas related to energy, human health and the environment. The research subjects include characterization and design of functional biological parts, processing systems and organisms, production of biomaterial and energy molecules through modification and synthesis of biosystems as well as innovation of synthetic biotechnology.

Research Groups:

Prof. Dr. Guo-Ping Zhao
Microbial Functional Genomics Group

Prof. Dr. Wei-Hong Jiang
Microbial Metabolic Regulation and Enzyme Engineering Group

Prof. Dr. Zhong-Jun Qin
Molecular Genetics of Actinomycetes and Genomic Engineering Group
　

Prof. Dr. Lai-Geng Li
Biomass and Bioenergy Group

Prof. Dr. Sheng Yang
Microbial Metabolic Regulation and Enzyme Engineering Group

Prof. Dr. Zhi-Hua Zhou
Microbial Metagenomics and Bioenergy Conversion Group
　

Prof. Dr. Chen Yang
Microbial Metabolic Engineering and Comparative Genomics Group

Prof. Dr. Peng Zhang
Cassava and Sweetpotato Biotechnology Group

 　

　

Academic Committee:
Sheng-Li Yang Academician Shanghai Research Center of Biotechnology, CAS
Zi-Xin Deng Academician Shanghai Jiao Tong University
Guo-Ping Zhao Academician Institute of Plant Physiology and Ecology, SIBS, CAS
Lu-Hua Lai Professor Peking University
Li Huang Professor Institute of Microbiology, CAS
Hai-Yan Liu Professor University of Science and Technology of China
Jia-Rui Wu Professor Shanghai Institutes of Biological Sciences, CAS
Si-Liang Zhang Professor East China University of Science and Technology
Dai-Jie Chen Professor Shanghai Institute of Pharmaceutical Industry
Lei Wang Professor Nankai University
Zhi-Hao Sun Professor Jiangnan University
Yi-Xue Li Professor Shanghai Center for Bioinformation Technology
Wen Liu Professor Shanghai Institute of Organic Chemistry, CAS
Wei-Hong Jiang Professor Institute of Plant Physiology and Ecology, SIBS, CAS
Hong-Wei Xue Professor Institute of Plant Physiology and Ecology, SIBS, CAS

Science Advisory Board:
Lu Yu Academician Institute of Theoretical Physics, CAS
Yun-Yu Shi Academician University of Science and Technology of China
Guo-Qiang Lin Academician Shanghai Institute of Organic Chemistry, CAS
Li-He Guo Professor Institute of Biochemistry and Cell Biology, SIBS, CAS
Zhang-Cheng Tang Professor Institute of Plant Physiology and Ecology, SIBS, CAS

Publications:

  1. Richard Sayre*, John Beeching, Ed Cahoon, Chiedozie Egesi, Claude Fauquet, John Fellman, Martin Fregene, Wilhelm Gruissem, Sally Mallowa, Mark Manary, Bussie Maziya-Dixon, Ada Mbanaso, Daniel P. Schachtman, Dimuth Siritunga, Nigel Taylor, Herve Vanderschuren, Peng Zhang (2011) The BioCassava Plus Program: Biofortification of cassava for sub-Saharan Africa. Annu Rev Plant Biol. 62: 251-272.

  2. Yang Liu, Haiming Xu, Suchao Chen, Xianfeng Chen, Zhenguo Zhang, Zhihong Zhu, Xueying Qin, Landian Hu, Jun Zhu, Guoping Zhao, Xiangyin Kong* (2011) Genome-Wide Interaction-Based Association Analysis Identified Multiple New Susceptibility Loci for Common Diseases. PLoS Genet. 7(3): e1001338.

  3. Qiang Gao, Kai Jin, Shenghua Ying, Yongjun Zhang, Guohua Xiao, Yanfang Shang, Zhibing Duan, Xiao Hu, Xueqin Xie, Gang Zhou, Guoxiong Peng, Zhibing Luo, Wei Huang, Bing Wang, Weiguo Fang, Sibao Wang, Yi Zhong, Lijun Ma, Raymond J St Leger, Guoping Zhao, Yan Pei, Mingguang Feng*, Yuxian Xia* and Chengshu Wang* (2011) Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum. PLoS Genet. 7(1): e1001264.

  4. Yi Zhong, Xiao Chang, Xingjun Cao, Yan Zhang, Huajun Zheng, Yongzhang Zhu, Chengsong Cai, Zelin Cui, Yunyi Zhang, Yuanyuan Li, Guoping Zhao, Shengyue Wang*, Yixue Li*, Rong Zeng*, Xuan Li*, Xiaokui Guo* (2011) Comparative Proteogenomic Analysis of the Leptospira interrogans Virulence Attenuated Strain IPAV against the Pathogenic Strain 56601. Cell Res. 21(8): 1210-1229.

  5. Jinshan Gui, Junhui Shen, and Laigeng Li* (2011) Functional Characterization of Evolutionarily Divergent 4-Coumarate:Coenzyme A Ligases in Rice. Plant Physiol. 157(2): 574-586.

  6. Semen A. Leyn, Xiaoqing Li, Qingxiang Zheng, Pavel S. Novichkov, Samantha Reed, Margaret F. Romine, James K. Fredrickson, Chen Yang, Andrei L. Osterman, and Dmitry A. Rodionov* (2011) Control of proteobacterial central carbon metabolism by the HexR transcriptional regulator: a case study in Shewanella oneidensis. J Biol Chem. 286(41): 35782–35794.

  7. Jun Yin, Zixin Deng, Guoping Zhao and Xi Huang* (2011) The N-terminal nucleophile serine of cephalosporin acylase executes the second autoproteolytic cleavage and acylpeptide hydrolysis*. J Biol Chem. 286(27): 24476-24486.

  8. Zhoutong Sun, Yuanyuan Ning, Lixia Liu, Yingmiao Liu, Bingbing Sun, Weihong Jiang, Chen Yang, Sheng Yang* (2011) Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. Microb Cell Fact. 10(1): 71.

  9. Pei Hao, Huajun Zheng, Yao Yu, Guohui Ding, Wenyi Gu, Shuting Chen, Zhonghao Yu, Shuangxi Ren, Munehiro Oda, Tomonobu Konno, Shengyue Wang, Xuan Li*, ZaiSi Ji* and Guoping Zhao* (2011) Complete Sequencing and Pan-Genomic Analysis of Lactobacillus delbrueckii subsp. bulgaricus Reveal Its Genetic Basis for Industrial Yogurt Production. PLoS ONE. 6(1): e15964.

  10. Jun Zhang, Yongjun Wei, Wei Xiao, Zhihua Zhou, Xing Yan* (2011) Performance and spatial community succession of an anaerobic baffled reactor treating acetone–butanol–ethanol fermentation wastewater. Bioresour Technol. 102: 7407–7414.

  11. Dongliang Song, Wang Xi, Junhui Shen,Ting Bi, Laigeng Li* (2011) Characterization of the plasma membrane proteins and receptor-like kinases associated with secondary vascular differentiation in poplar. Plant Mol Biol. 76(1-2): 97–115.

  12. Shiyuan Hu, Huajun Zheng, Yang Gu, Jingbo Zhao, Weiwen Zhang, Yunliu Yang, Shengyue Wang, Guoping Zhao, Sheng Yang* and Weihong Jiang* (2011) Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018. BMC Genomics. 12: 93.

  13. Ruofan Wang, Wenming Zong, Changli Qian, Yongjun Wei，Ruisong Yu, Zhihua Zhou* (2011) Isolation of Clostridium perfringens strain W11 and optimization of its biohydrogen production by genetic modification. Int J. Hydrogen Energy. 36: 12159-12167.

  14. Ning Liu, Xing Yan, Meiling Zhang, Lei Xie, Qian Wang,Yongping Huang, Xuguo Zhou, Shengyue Wang, Zhihua Zhou* (2011) Microbiome of fungus-growing termites: a new reservoir for mining lignocellulase genes. Appl Environ Microbiol. 77: 48-56.

  15. Han Xiao, Yang Gu, Yuanyuan Ning, Yunliu Yang, Wilfrid J. Mitchell, Sheng Yang*, and Weihong Jiang* (2011) Confirmation and Elimination of Xylose Metabolism Bottlenecks in Glucose Phosphoenolpyruvate-Dependent Phosphotransferase System-Deficient Clostridium acetobutylicum for Simultaneous Utilization of Glucose, Xylose, and Arabinose. Appl Environ Microbiol. 77(22): 7886-7895.

  16. Hui Dong*, Hongyi Zhang, Jianping Liang, Huadong Yan, Yangyi Chen, Yan Shen, Yalin Kong, Shengyue Wang, Guoping Zhao and Weirong Jin (2011) Digital karyotyping reveals probable target genes at 7q21.3 locus in hepatocellular carcinoma. BMC Med Genomics. Epub ahead of print.

  17. Yongzhen Tao, Deng Liu, Xing Yan, Zhihua Zhou, Jeong K. Lee, and Chen Yang* (2011) Network identification and flux quantification of glucose metabolism in Rhodobacter sphaeroides under photoheterotrophic H2-producing conditions. J. Bacteriol. Accepted.

  18. Yinhua Lu, Juanmei He, Hong Zhu, Zhenyu Yu, Rui Wang, Yunliang Chen, Fujun Dang, Weiwen Zhang, Sheng Yang, and Weihong Jiang* (2011) An Orphan Histidine Kinase, OhkA, Regulates Both Secondary Metabolism and Morphological Differentiation in Streptomyces coelicolor. J. Bacteriol. 193(12): 3020-3032.

  19. Lei Zhang, Semen Leyn, Yang Gu, Weihong Jiang, Dmitry Rodionov*, and Chen Yang* (2011) Transcriptional Regulation of Arabinose Metabolism in Clostridium acetobutylicum: Comparative Genomic Reconstruction and Experimental Characterization of AraR Regulon. J. Bacteriol. Accepted.

  20. Fuchao Li, Peng Jiang, Huajun Zheng, Shengyue Wang, Guoping Zhao, Song Qin*, and Zhaopu Liu* (2011) Draft Genome Sequence of the Marine Bacterium Streptomyces griseoaurantiacus M045, Which Produces Novel Manumycin-Type Antibiotics with a pABA Core Component. J. Bacteriol. 193(13): 3417-3418.

  21. Shanshan Zhao, Dominique Dufour, Teresa Sánchez, Hernan Ceballos, Peng Zhang* (2011) Development of waxy cassava with different biological and physico-chemical characteristics of starches for industrial applications. Biotechnol Bioeng. 108(8): 1925-1935.

  22. Li Zhu, Rongsheng Tao, Yi Wang, Yu Jiang, Xin Lin,Yunliu Yang, Huabao Zheng, Weihong Jiang, Sheng Yang* (2011) Removal of L: -alanine from the production of L: -2-aminobutyric acid by introduction of alanine racemase and D: -amino acid oxidase. Appl Microbiol Biotechnol. 90(3):903-910.

  23. Dalong Zhang, Fuyun Zhu, Wenchao Fan, Rongsheng Tao, Hong Yu, Yunliu Yang, Weihong Jiang, Sheng Yang* (2011) Gradually accumulating beneficial mutations to improve the thermostability of N-carbamoyl-D: -amino acid amidohydrolase by step-wise evolution. Appl Microbiol Biotechnol. 90(4): 1361-1371.

  24. Qiongyi Zhao, Yi Wang, Yimeng Kong, Da Luo, Xuan Li* and Pei Hao* (2011) Optimizing de novo transcriptome assembly fom short-read RNA-Seq data: a comparative study. BMC Bioinformatics. 12(Suppl 14): S2.

  25. Weihua Chen and Zhongjun Qin* (2011) Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters. BMC Microbiol. 11(1): 243.

  26. Bikui Tang, Ruihua Zhao, Yong Sun, Ying Zhu, Jiang Zhong, Guoping Zhao and Naishuo Zhu* (2011) Interleukin-6 expression was regulated by epigenetic mechanisms in response to influenza virus infection or dsRNA treatmen. Mol Immunol. 48(8): 1001-1008.

  27. Jun Yang, Hui-Ping Bi, Wei-Juan Fan, Min Zhang, Hong-Xia Wang, Peng Zhang*. (2011) Efficient embryogenic suspension culturing and rapid transformation of a range of elite genotypes of sweet potato (Ipomoea batatas [L.] Lam.). Plant Science. 181: 701-711.

  28. Liang Ma, Jun Zhang, Gen Zou, Chengshu Wang, Zhihua Zhou* (2011) Improvement of cellulase activity in Trichoderma reesei by heterologous expression of a beta-glucosidase gene from Penicillium decumbens. Enzyme Microb. Technol. 49(4): 366-371.

  29. Wenqian Wei, Fangqi Liu, Lei Liu, Zuofeng Li, Xiaoyan Zhang, Fan Jiang, Qu Shi, Xiaoyan Zhou, Weiqi Sheng, Sanjun Cai, Xuan Li*, Ye Xu*, Peng Nan* (2011) Distinct mutations in MLH1 and MSH2 genes in hereditary non-polyposis colorectal cancer (HNPCC) families from China. BMB Rep. 44(5): 317-322.

  30. Jingfang Wang*, Pei Hao, Yixue Li, Jianliang Dai, Xuan Li (2011) Exploration of conformational transition in the aryl-binding site of human FXa using molecular dynamics simulations. J Mol Model. DOI 10.1007/s00894-011-1295-x.

  31. Lida Yang, Zhongmei Chu, Yi Zhang*, Shengli Yang (2011) Gly-345 plays an essential role in Pyrococcus furiosus chaperonin function. Biotechnol Lett. 33(8): 1649–1655.

  32. Zhihui Shao, Jin Gao, Xiaoming Ding, Jin Wang, Juishen Chiao, Guoping Zhao* (2011) Identification and functional analysis of a nitrate assimilation operon nasACKBDEF from Amycolatopsis mediterranei U32. Arch Microbiol. 193(7): 463-477.

  33. C. Wang, Z. Lentini, E. Tabares, M. Quintero, H. Ceballos*, B. Dedicova, C. Sautter, C. Olaya, P. Zhang (2011) Microsporogenesis and pollen formation in cassava. Biol Plantarum. 55(3): 469-478.

  34. Jun Yang, Dong An, Peng Zhang* (2011) Expression profiling of cassava storage roots reveals an active process of glycolysis/gluconeogenesis. J Integr Plant Biol. 53(3): 193-211.

  35. Jia Liu, Qijie Zheng, Qiuxiang Ma, Kranthi Kumar Gadidasu, Peng Zhang* (2011) Cassava genetic transformation and its application in breeding. J Integr Plant Biol. 53(7): 552-569.

  36. Qing Zhang, Yunyi Zhang, Yi Zhong, Jun Ma, Nanqiu Peng, Xingjun Cao, Chen Yang, Rong Zeng, Xiaokui Guo and Guoping Zhao* (2011) Leptospira interrogans encodes an ROK family glucokinase involved in a cryptic glucose utilization pathway. Acta Biochim Biophys Sin (Shanghai). 43(8): 618-29.

  37. Peng Guo, Qiuxiang Cheng, Pegnfei Xie, Yun Fan, Weihong Jiang, and Zhongjun Qin* (2011) Characterization of the multiple CRISPR loci on Streptomyces linear plasmid pSHK1. Acta Biochim Biophys Sin (Shanghai). 43(8): 630-639.

  38. Hui Dong, Yangyi Chen, Yan Shen, Shengyue Wang, Guoping Zhao* and Weirong Jin* (2011) Artificial duplicate reads in sequencing data of 454 Genome Sequencer FLX System. Acta Biochim Biophys Sin (Shanghai). 43 (6): 496-500.

  39. Ana Zeng, Tao Wang, Haiyang Xia, Shiyuan Peng, Weihua Chen, Chaneglin Jiang, Lihua Xu, Li Zhong, Meijuan Shen*, and Zhongjun Qin* (2011) Development of a vector and host system and characterization of replication of plasmid pSQ10 in moderately halophilic Nocardiopsis. Acta Biochim Biophys Sin (Shanghai). 43(9): 738-743.

  40. Huan Yuan, Wei Zhao, Yi Zhong, Jin Wang, Zhongjun Qin, Xiaoming Ding and Guoping Zhao* (2011) Two genes, rif15 and rif16, of the rifamycin biosynthetic gene cluster in Amycolatopsis mediterraneilikely encode a transketolase and a P450 monooxygenase, respectively, bothessential for the conversion of rifamycin SV into B. Acta Biochim Biophys Sin (Shanghai). 43(12): 948-956.

  41. Mahender Aileni, Sadanandam Abbagani, Peng Zhang* (2011) Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: Plant regeneration via shoot organogenesis. Plant Biotechnol Rep. 5: 147-156.

  42. Chen Feifei, Lin Ling, Wang Lu, Tan Yi, Zhou Hongxia, Wang Yiguang, Wang Yong*, He Weiqing*. (2011) Distribution of dTDP–glucose-4,6 dehydratase genes and diversity of potential glycosylated natural products in marine bacteria. Appl Microbiol Biotechnol. 90(4): 1347-1359.

  43. Hailin Meng, Zhiguo Lu, Yong Wang*, Siliang Zhang, Xiaoning Wang (2011) In silico analysis of erythromycin precursor 6-deoxyerythronolide B by heterologous biosynthesis in Escherichia coli. Biotechnol and Bioproc E. 16(3): 445-456.

  44. Hailin Meng, Yong Wang*, Siliang Zhang, Xiaoning wang (2011) In silico analysis and experimental improvement of the terpenoid precursor supply pathways for taxadiene heterologous biosynthesis in Escherichia coli. Biotechnol and Bioproc E. 16 (2): 205-215.

  45. Yongcong Tan, Qijun Wang, Guoping Zhao* and Yufeng Yao* (2011) Protein post-translational modification in prokaryotes. Prog Biochem Biophys. 38(3): 197-203.

  46. Yun Li, Kang Tu, Siyuan Zheng, JingfangI Wang, Yixue Li, Pei Hao, Xuan Li* (2011) Association of feature gene expression with structural fingerprints of chemical compounds. J Bioinform Comput Biol. 9(4): 503-519.

  47. Yang Gu, Yu Jiang, Hui Wu, Xudong Liu, Zhilin Li, Jian Li, Han Xiao, Zhaobing Shen, Hongjun Dong, Yunliu Yang, Yin Li, Weihong Jiang* and Sheng Yang* (2011) Economical challenges to microbial producers of butanol: Feedstock, butanol ratio and titer. Biotechnol J. 6(11): 1348-57.

  48. Lin Zhang, Guoping Zhao*, Xiaoming Ding* (2011) Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination. Scientific Reports. 1, 141.

  49. Hou Shen, Laigeng Li (2011) Rapid characterization of woody biomass digestibility and chemical composition using Near-infrared Spectroscopy. J. Integr. Plant Biol. 53(2): 166-175.

  50. 于瑞嵩，宗文明，周志华* (2011) 异源表达【FeFe】氢酶的基因工程大肠杆菌的构建. 微生物学报. 51(11): 1468-1475.

  51. 王钱福，严兴，魏维，张磊，钱昌丽，周志华* (2011).生物元件的挖掘、改造与标准化. 生命科学. 23: 860-868.

  52. 赵运军，李来庚* (2011) 植物细胞壁松弛因子. 植物生理学报. 47 (10): 925-935.

  53. 陈菲菲，王勇*，王以光，赫卫清* (2011) 海洋微生物来源的天然产物开发研究进展. 应用与环境生物学报. 17(2): 287-294.

  54. 林灵，谭亿，陈菲菲，周红霞，王以光，赫卫清*，王勇* (2011) 大连渤海老虎滩海域沉积物可培养放线菌的多样性研究. 微生物学报. 51(2): 262-269.

  55. 陈菲菲，林灵，王以光，周红霞，陶佩珍，赫卫清*，王勇* (2011) 海洋细菌活性化合物的筛选策略. 中国抗生素杂志. 36(5): 321-330.

  56. 蒙海林，汪建峰，王勇*，张嗣良，王小宁 (2011) CYP725A4分子序列分析与结构建模. 华南理工大学学报. 39(5): 154-159.

  57. 卢志国，汪建峰，蒙海林，熊智强，王勇* (2011) 合成生物学与天然产物开发. 生命科学. 23(9): 900-911.

  58. Hui Dong, Yanjun Zhang, Hui Xiong, An Yan, Guohui Ding, Yangyi Chen, Liqun Xie, Jiazheng Chen, Guoqing Zhang, Pei Hao, Liming Cong, Yiyu Lu, Xiaoyan Che, Xiaoning Wang, Yixue Li, Kwok-Yung Yuen, Guoping Zhao, and Weirong Jin*. (2010). Detection of human novel influenza A (H1N1) viruses using multi-fluorescent real-time RT-PCR. Virus Res 147(1): 85-90.

  59. Qijun Wang, Yakun Zhang, Chen Yang, Hui Xiong, Yan Lin, Jun Yao, Hong Li, Lu Xie, Wei Zhao, Yufeng Yao, Zhi-Bin Ning, Rong Zeng, Yue Xiong, Kun-Liang Guan, Shimin Zhao*, Guo-Ping Zhao*. (2010). Acetylation of metabolic enzymes coordinates carbon source utilization and metabolic flux. Science 327: 1004-1007.

  60. Wei Zhao, Yi Zhong, Hua Yuan, Jin Wang, Huajun Zheng, Ying Wang, Xufeng Cen, Feng Xu, Jie Bai, Xiaobiao Han, Gang Lu, Yongqiang Zhu, Zhihui Shao, Han Yan, Chen Li, Nanqiu Peng, Zilong Zhang, Yunyi Zhang, Wei Lin, Yun Fan, Zhongjun Qin, Yongfei Hu, Baoli Zhu, Shengyue Wang*, Xiaoming Ding* and Guo-Ping Zhao*. (2010). Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism. Cell Res 20: 1096-1108.

  61. Liang-dong Lu , Qing Sun, Xiao-yong Fan, Yi Zhong, Yu-feng Yao*, Guo-Ping Zhao*. (2010). Mycobacterial MazG is a novel NTP pyrophosphohydrolase involved in oxidative stress response. J Biol Chem 285(36): 28076-85.

  62. Yan-Xia Wei, Zhuo-Yang Zhang, Chang Liu, Yong-Zhang Zhu, Yong-Qiang Zhu, Huajun Zheng, Guo-Ping Zhao, Shengyue Wang, and Xiao-Kui Guo*. (2010). Complete genome sequence of Bifidobacterium longum JDM301. J Bacteriol 192 (15): 4076-7.

  63. Ke Wang, Xiao-Hui Xiang, Fei He, Li-Bo Lin, Rong Zhang, Xing-Jie Ping, Ji-Sheng Han, Ning Guo, Qing-Hua Zhang, Cai-Lian Cui, Guo-Ping Zhao*. (2010). Transcriptome profiling analysis reveals region-distinctive changes of gene expression in the CNS in response to different moderate restraint stress. J Neurochem 113(6): 1436-46.

  64. Cheng-Song Cai, Yong-Zhang Zhu, Yi Zhong, Xiao-Fang Xin, Xiu-Gao Jiang, Xiao-Li Lou, Ping He, Jin-Hong Qin, Guo-Ping Zhao, Sheng-Yue Wang and Xiao-Kui Guo*. (2010). Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China. BMC Microbiol 10: 67.

  65. Xing-Jun Cao, Jie Dai, Hao Xu, Song Nie, Xiao Chang, Bao-Yu Hu, Quan-Hu Sheng, Lian-Shui Wang, Zhi-Bin Ning, Yi-Xue Li, Xiao-Kui Guo, Guo-Ping Zhao and Rong Zeng*. (2010). High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans. Cell Res 20(2): 197-210.

  66. Zuoquan Xie, Houkai Li, Ke Wang, Jingchao Lin, Qi Wang, Guoping Zhao, Wei Jia*, and Qinghua Zhang*. (2010). Analysis of transcriptome and metabolome profiles alterations in fatty liver induced by high-fat diet in rat. Metabolism 59(4): 554-60.

  67. Junwei Yang, Jing Xu, Xiaoying Chen, Yixuan Zhang, Xucheng Jiang, Xiaokui Guo, Guoping Zhao*. (2010). Decrease of plasma platelet-activating factor acetylhydrolase activity in lipopolysaccharide induced mongolian gerbil sepsis model. PLoS One 5(2): e9190.

  68. Ke Dong, Qingtian Li, Chang Liu, Yunyi Zhang, Guoping Zhao, and Xiaokui Guo*. (2010). Cloning and characterization of three cheB genes in Leptospira interrogans. Acta Biochim Biophys Sin (Shanghai) 42(3): 216-23.

  69. Xinfeng Li, Tong Liu, Yongqiang Wu, Guoping Zhao, Zhihua Zhou*. (2010). Derepressive effect of NH4+ on hydrogen production by deleting the glnA1 gene in Rhodobacter sphaeroides. Biotechnol Bioeng 106(4): 564-72.

  70. Xianfeng Chen, Xinlei Li, Ping Wang, Yang Liu, Zhenguo Zhang, Guoping Zhao, Haiming Xu, Jun Zhu, Xueying Qin, Suchao Chen, Landian Hu, Xiangyin Kong*. (2010). Novel association strategy with copy number variation for identifying new risk Loci of human diseases. PLoS One 5(8) pii: e12185.

  71. Lin Zhang, Lu Wang, Jin Wang, Xijun Ou, Guoping Zhao*, Xiaoming Ding*. (2010). DNA cleavage is independent of synapsis during Streptomyces phage fBT1 integrase-mediated site-specific recombination. J Mol Cell Biol 2: 264-275.

  72. Gu Yang, Yi Ding, Cong Ren, Zhe Sun, Dmitry A Rodionov, Weiwen Zhang, Sheng Yang, Chen Yang* and Weihong Jiang*. (2010). Reconstruction of xylose utilization pathway and regulons in Firmicutes. BMC Genomics 11: 255-68.

  73. Ren Cong, Yang Gu, Shiyuan Hu, Yan Wu, Pan Wang, Yunliu Yang , Chen Yang, Sheng Yang*, and Weihong Jiang*. (2010). Identification and inactivation of pleiotropic regulator CcpA to eliminate glucose repression of xylose utilization in Clostridium acetobutylicum. Metabolic Engineering 12(5): 446-454.

  74. Li Jian, Lijun Zhao, Meng Zhao, Yunliu Yang, Weiwen Zhang, Sheng Yang*. (2010). Screening and characterization of butanol tolerant microorganisms. Letters in Applied Microbiology 50: 373-379.

  75. Hu Shiyuan, Jun Chen, Zhongyi Yang, Lijun Shao, Hua Bai, Jiali Luo, Weihong Jiang, Yunliu Yang*. (2010). Coupled bioconversion for preparation of N-acetyl-Dneuraminic acid using immobilized N-acetyl-D-glucosamine-2-epimerase and N-acetyl-Dneuraminic acid lyase. Applied Microbiology and Biotechnology 85(5): 1383-91.

  76. Li Zhong, Qiuxiang Cheng, Xinli Tian, Liqian Zhao, and Zhongjun Qin*. (2010). Characterization of the Replication, Transfer and Plasmid/Lytic Phage Cycle of the Streptomyces Plasmid-Phage pZL12. Journal of Bacteriolog. 192: 3747-3754.

  77. Ran Zhang, Shiyuan Peng, and Zhongjun Qin*. (2010). Two Internal Origins of Replication on Streptomyces Linear Plasmid pFRL1. Applied and Environmental Microbiology 76(17): 5676-5683.

  78. Zhiqun Lu, Pengfei Xie, and Zhongjun Qin*. (2010). Promotion of markerless deletion of the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor. Acta Biochimica et Biophysica Sinica 42(10): 717-721.

  79. Ning Liu, Xing Yan, Meiling Zhang, Lei Xie, Qian Wang, Xuguo Zhou,Shengyue Wang, Zhihua Zhou*. (2010). Microbiome of fungus-growing termites: a new reservoir for mining lignocellulase genes. Applied and Environmental Microbiology 77: 48-56.

  80. Tong Liu, Xinfeng Li, Zhihua Zhou*. (2010). Improvement of hydrogen yield by hupr gene knock-out and nifa gene overexpression in Rhodobacter sphaeroides 6016. International Journal of Hydrogen Energy 35: 9603-9610.

  81. Yue Huang, Wenming Zong, Xing Yang, Ruofan Wang, Christopher L. Hemme, Jizhong Zhou, Zhihua Zhou*. (2010). Succession of the bacterial community and dynamics of hydrogen producers in a hydrogen-producing bioreactor. Applied and Environmental Microbiology 76: 3387-3390.

  82. Qin Sun, Wei Xiao, Dan Xin, Jiping Shi, Xing Yan, Zhihua Zhou*. (2010). Statistical optimization of process parameters on biohydrogen production from sucrose by co-culture of Clostridium acidisoli sp. Nov and Rhodobacter sphaeroides. International Journal of Hydrogen Energy 35: 4076-4084.

  83. Alei Geng, Yanling He, Changli Qian, Xing Yan and Zhihua Zhou*. (2010). Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and C. thermopalmarium. Bioresource Technology 101(11): 4029-4033.

  84. Wei Xiong, Lixia Liu, Chao Wu, Chen Yang*, Qingyu Wu*. (2010). 13C tracer and GC-MS analysis reveals metabolic flux distribution in oleaginous microalga Chlorella protothecoides. Plant Physiol. 154: 1001-1011.

  85. Dmitry A Rodionov, Chen Yang, Xiaoqing Li, Irina A Rodionova, Yanbing Wang, Anna Y Obraztsova, Olga P Zagnitko, Ross Overbeek, Margaret F Romine, Samantha Reed, James K Fredrickson, Kenneth H Nealson, Andrei L Osterman*. (2010). Genomic encyclopedia of sugar utilization pathways in the Shewanella genus. BMC Genomics 11: 494.

  86. Shanfa Lu, Laigeng Li, Gongke Zhou. (2010). Genetic modification of wood quality for second-generation biofuel production. GM Crops 4: 1-7.

  87. Dongliang Song, Junhui Shen, Laigeng Li. (2010). Characterization of cellulose synthase complexes in Populus xylem differentiation. New Phytologist 187: 777-790.

  88. Horvath, B., Peszlen, I., Peralta, P., Horvath. L, Kasal, B., & Li, L. (2010). Elastic modulus determination of transgenic aspen trees using a dynamic mechanical analyzer in static bending mode. Forest Products J 60(3): 296-300.

  89. Horvath, B., Peralta, P., Peszlen, I., Divos, F., Kasal, B., & Li, L. (2010). Elastic modulus of transgenic aspen. Wood Research 55(1):1-10.

  90. Horvath, B., Peszlen,I., Peralta,P., Kasal, B. and Li, L. (2010). Effect of lignin genetic modification on wood anatomy of aspen trees. IAWA J 31: 29-38.

  91. Suzuki, S., Sakakibara, N., Li, L., Umezawa, T. and Chiang, V. L. (2010). Profiling of phenylpropanoid monomers in developing xylem tissue of transgenic aspen (Populus tremuloides). J. Wood Sci. 56: 71-76.

  92. Huiping Bi, Mahender Aileni, Peng Zhang*. (2010). Evaluation of cassava varieties for cassava mosaic disease resistance jointly by agro-inoculation screening and molecular markers. Afr. J. Plant Sci 4(9): 330-3.

  93. Peng Zhang*, Wen-Quan Wang, Gen-Liang Zhang, Miroslav Kaminek, Petre Dobrev, Jia Xu, and Wilhelm Gruissem. (2010). Senescence-inducible expression of isopentenyl transferase extends leaf life, increases drought stress resistance and alters cytokinin metabolism in cassava. J. Integr. Plant Biol 52(7): 653-669.

  94. Jia Xu, Mahender Aileni, Sadanandam Abbagani, Peng Zhang*. (2010). A reliable and efficient method for total RNA isolation from various members of spurge family (Euphorbiaceae). Phytochem. Anal 21(5): 395-398.

  95. Yao Yu, Tao Xu, Yongtao Yu, Pei Hao*, Xuan Li*. (2010). Association of tissue lineage and gene expression: conservatively and differentially expressed genes define common and special functions of tissues. BMC Bioinformatics 11(Suppl 11):S1

  96. Tao Xu, Jie Ping, Yao Yu, Fudong Yu, Yongtao Yu, Pei Hao*, Xuan Li*. (2010). Revealing parasite influence in metabolic pathways in Apicomplexa infected patients. BMC Bioinformatics 11(Suppl 11):S13

  97. Xiang Tang, Zhi-Yong Zhang, Wen-Juan Zhang, Xing-Ming Zhao, Xuan Li, Dong Zhang, Qiao-Quan Liu, and Wei-Hua Tang*. (2010). Global gene profiling of laser-captured pollen mother cells indicates molecular pathways and gene subfamilies involved in rice meiosis. Plant Physiol 154(4): 1855-70.

  98. Hui Chen, Lida Yang, Yi Zhang*, Shengli Yang. (2010). Over-expression and characterization of the recombinant prefoldin from hyperthermophilic archaeum Pyrococcus furiosus. Biotechnology Letters 32(3): 429-434.

  99. 顾阳, 蒋宇, 吴辉, 刘旭东, 李治林, 李键, 肖晗, 沈兆兵, 赵静波, 杨蕴刘, 姜卫红, 杨晟. (2010). 生物丁醇制造技术现状和展望. 生物工程学报. 科学出版社. July 25. 26(7): 1-10.

  100. 杨俊杰, 范文超, 肖晗, 关春鸿, 曹传增, 邵海峰, 姜卫红, 杨晟. (2010) 枯草芽胞杆菌基因组混组方法初探. 生物工程学报. 科学出版社. October 25. 26(10): 1385-1392.

  101. 田新莉, 钟莉, 成秋香, 陈振华, 周敏, 王韬, 范云, 杨勇, 郭鹏, 夏海洋, 覃重军. (2010). 从87种中草药植物中分离内生放线菌及其规律的初探. 中国抗生素杂志 35(9): 659-662.

  102. 朱颖旻, 许铭翾, 沈美娟, 陈振华, 覃重军. (2010). 红球菌线型质粒pNSL1的克隆、测序和复制区的鉴定. 微生物学报 50(8): 1098-1103.

  103. 杨勇, 代玉梅, 陈振华, 覃重军. (2010). 五个链霉菌线型质粒端粒的克隆和分析. 微生物学报 50(8): 1008-1013.

  104. 杨勇, 钟莉, 田新莉, 成秋香, 陈振华, 覃重军. (2010). 小葱植物内生链霉菌线型质粒pYY8L的端粒与复制区的克隆和鉴定. 微生物学报 50(4): 452-458.

  105. 张子飞, 陈威华, 覃重军. (2010). 高温链霉菌质粒pTSC2的测序分析和滚环复制方式的鉴定. 微生物学报 50(1): 41-47.

  106. 周志华, 严兴, 王钱福, 张磊, 张珺, 赵国屏. (2010). 生物资源的开发利用技术. 工业生物技术发展报告 55-66.

  107. 宋东亮, 沈君辉, 李来庚. (2010). 植物维管系统形成的调控机制. 植物生理通讯 46: 411-422.

  108. 沈君辉, 宋东亮, 赵运军, 李来庚. (2010). 桉树基因组和功能基因组. 植物生理通讯 46: 94-100.

  109. 刘桂玲, 郑建利, 柳新明, 张鹏*. (2010). 能源型甘薯新品种“泰中9号”高产栽培技术标准的研究. 中国农学通报 26(10): 122-127.

  110. 赵姗姗, 李海霞, 刘佳, 黄晨, 马秋香, 张鹏*. (2010). 我国主要栽培木薯品种体细胞胚胎发生与器官发生的比较研究. 农业生物技术学报 18(1): 37-44.

  111. Pinchuk, G.E., Rodionov, D.A., Yang, C., Li, X., Osterman, A., Dervyn, E., Geydebrekht, O.V., Reed, S.B., Romine, M.F., Collart, F.R., Scott, J.H., Fredrickson, J.K., Beliaev, A.S. (2009). Genomic reconstruction of Shewanella oneidensis MR-1 metabolism reveals a previously uncharacterized machinery for lactate utilization. Proc. Natl. Acad. Sci. USA. 106: 2874-2879.

  112. Yang J, Zhang Y, Xu J, Geng Y, Chen X, Yang H, Wang S, Wang H, Jiang X, Guo X, Zhao G. (2009) Serum activity of platelet-activating factor acetylhydrolase is a potential clinical marker for leptospirosis pulmonary hemorrhage. PloS one. 4(1):e4181.

  113. Xijun Ou, Bo Zhang, Lin Zhang, Guoping Zhao* and Xiaoming Ding* (2009) Characterization of rrdA, a TetR family protein gene involved in the regulation of secondary metabolism in Streptomyces coelicolor. Appl. Environ. Microbiol. 75(7): 2158-2165.

  114. Cai Y., P, Trodler, S. Jiang, W. Zhang, Y. Wu, Y. Lu, S. Yang, W. Jiang. (2009) Isolation and molecular characterization of a novel d-hydantoinase from Jannaschia sp. CCS1. FEBS Journal DOI: 10.1111/j.1742-4658.2009.07077.X

  115. J Yu., C. Xu. F. Dong, Y. Yang., W. Jiang*, S.Yang* (2009) Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metabolic Engineering. DOI: 10.1016/j.ymben.2009.06.002.

  116. Xie P., Zeng A., and Qin Z (2009) cmdABCDEF, a cluster of genes encoding membrane proteins for differentiation and antibiotic production in Streptoymyces coelicolor A3(2). BMC Microbiology. 9:157.

  117. Junjie YANG, Weihong JIANG, Sheng YANG* (2009). mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Res. 9(4):600-609.

  118. Yang Gu, Sheng YANG*, Weihong JIANG*.(2009) Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coli. Journal of Biotechnology, accepted

  119. Liu F.H, S.B. Wang, J.S. Zhang, J. Zhang, X. Yan, H.K. Zhou, G.P. Zhao and Z.H. Zhou. (2009) The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis. Journal of Applied Microbiology 106:952-966.

  120. Liu F.H, G.H. Lin, G. Gao, B.Q. Qin, J.S. Zhang, G.P. Zhao, Z.H. Zhou and J.H. Shen. (2009) Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis (DGGE). Journal of Applied Microbiology 106:1022-1032.

  121. Hong YU, Jian LI, Dalong ZHANG, Yunliu YANG, Weihong JIANG, Sheng YANG* (2009). Improving the thermostability of N-carbamyl-D-amino acid amidohydrolase by error-prone PCR. Appl. Microbiol. Biotechnol. 82(2) 279.

  122. Zhang R, Xia H, Guo P. and Qin Z (2009) Variation in the replication loci of Streptomyces linear plasmids. FEMS Microbiological Letter. 290:209-216.

  123. Wang W., D. Shu, L. Chen, W. Jiang, Y. Lu. (2009) Cross-talk between an orphan response regulator and a noncognate histidine kinase in Streptomyces coelicolor. FEMS Microbiol Lett 294:150-156.

  124. Fan XY, Ma H, Guo J, Li ZM, Cheng ZH, Guo SQ, Zhao GP* (2009) A novel differential expression system for gene modulation in Mycobacteria. Plasmid. 61(1): 39-46. Epub 2008 Oct 25.

  125. Tian Y., Jiang W., Zhao G., and Qin Z (2009) In vivo conjugation-coupled recombinational cloning of a Streptomyces lividans chromosomal telomeric DNA using a linear plasmid. Biotechnology Letter. 31(8):1253-1258.

  126. Shu D., L. Chen, W. Wang, Z. Yu, C. Ren, W. Zhang, S. Yang, Y. Lu*, W. Jiang*. (2009) afsQ1-Q2-sigQ is a pleiotropic but conditionally required signal transduction system for both secondary metabolism and morphological development in Streptomyces coelicolor. Applied Microbiology and Biotechnology 81:1149-1160.

  127. Yu H., J. Li, D. Zhang, Y. Yang, W. Jiang, S.Yang*. (2009) Improving the thermostability of N-Carbamyl-D-amino acid amidohydrolase by error-prone PCR. Applied Microbiology and Biotechnology 82:279-285.

  128. Gu Y., S. Hu, J. Chen, L. Shao , H. He , Y. Yang , S. Yang*, W. Jiang*.(2009) Ammonium acetate enhances solvent production by Clostridium acetobutylicum EA 2018 using cassava as a fermentation medium. Journal of Industrial Microbiology and Biotechnology. DOI: 10.1007/s10295-009-0604-1.

  129. Hu S., J. Chen, Z.Yang, L. Shao, H. Bai, J. Luo,W. Jiang, Y. Yang* .(2009) Coupled bioconversion for preparation of N-acetyl-Dneuraminic acid using immobilized N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid lyase, Applied Microbiology and Biotechnology. DOI 10.1007/s00253-009-2163-9.

  130. 刘桂玲, 郑建利, 柳新明, 陈宝芳, 张鹏. (2009) 不同基因型甘薯品种产量与地上部农艺性状及主要气象因子关联度分析. 中国农学通报. 25(05):125-131.

  131. 胡世元, 杨蕴刘, 杨晟, 姜卫红. (2009) 丙酮丁醇梭菌的特性及遗传改造. 工业生物技术发展报告. 科学出版社 94-103.

  132. Chen L, Lu Y, Chen J, Zhang W, Shu D, Qin Z, Yang, S and Jiang W. (2008) Characterization of a negative regulator AveI for avermectin biosynthesis in Streptomyces avermitilis NRRL8165. Applied Microbiolology and Biotechnology 80:277-286.

  133. Min Li, Baohong Wang, Menghui Zhang, Mattias Rantalainen, Shengyue Wang, Haokui Zhou, Yan Zhang, Jian Shen, Xiaoyan Pang, Meiling Zhang, Hua Wei, Yu Chen†, Haifeng Lu, Jian Zuo, Mingming Su, Yunping Qiu, Wei Jia, Chaoni Xiao, Leon M. Smith, Shengli Yang, Elaine Holmes, Huiru Tang, Guoping Zhao*, Jeremy K. Nicholson, Lanjuan Li, and Liping Zhao (2008) Symbiotic gut microbes modulate human metabolic phenotypes. Proc. Natl. Acad. Sci. USA. 105: 2117-2122.

  134. Jun MA, Peng ZHANG, Zilong ZHANG, Manwu ZHA, Hai XU, Guoping ZHAO* and Jianping DING (2008) Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase from Leptospira interrogans. Biochem. J. 415, 45-56.

  135. Xiaonan Yang, Hongliang Yang, Gangqiao Zhou, and Guo-Ping Zhao* (2008) Infectious Disease in the Genomic Era. Annu. Rev. Genomics Hum. Genet. 9:21-48.

  136. Lin Zhang, Xijun Ou, Guoping Zhao*, and Xiaoming Ding* (2008) Highly efficient in vitro site-specific recombination system based on Streptomyces phage BT1 integrase. J. Bacteriol. 190: 6392-6397.

  137. Xijun Ou, Bo Zhang, Lin Zhang, Kai Dong, Chun Liu, Guoping Zhao*, and Xiaoming Ding* (2008) SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145. Acta Biochim Biophys Sin 40: 877-882.

  138. Qin JH, Zhang Q, Zhang ZM, Zhong Y, Yang Y, Hu BY, Zhao GP, Guo XK. (2008) Identification of a novel prophage-like gene cluster actively expressed in both virulent and avirulent strains of Leptospira interrogans serovar lai. Infect Immun. 76(6): 2411-9.

  139. Zhang YX, Geng Y, Yang JW, Guo XK, Zhao GP. (2008) Cytotoxic activity and probable apoptotic effect of Sph2, a sphigomyelinase hemolysin from Leptospira interrogans strain Lai. BMB Rep. 41(2): 119-25.

  140. Zhang R., Zeng A., Fang P., and Qin Z (2008) Characterization of the replication loci of Streptomyces circular plasmids pFP11 and pFP1 and their ability to propagate in linear mode with artificially attached telomeres. Applied and Environmental Microbiology. 74(11):3368-3376.

  141. Zhao J., Zhong L., Shen M., Xia Z., Cheng Q., Sun X., Zhao G., Li Y., and Qin Z (2008) Discovery of an autonomously-replicating plasmid pMF1 from Myxococcus fulvus and the development of a gene cloning system in Myxococcus Xanthus. Applied and Environmental Microbiology. 74(7):1980-1987.

  142. Tao Y, et al. and Zhou Z 2008 Characteristics of a new photosynthetic bacterial strain for hydrogen production and its application in wastewater treatment. International Journal of Hydrogen Energy 33: 963-973.

  143. Yang C, Rodionov DA, Rodionova IA, Li X, Osterman A. (2008) Glycerate 2-kinase family and genomic reconstruction of related metabolic pathways. Case study in Thermotoga maritima. Journal of Bacteriology 190: 1773-1782.

  144. Knowles LM, Yang C, Osterman A, Smith JW. (2008) Inhibition of fatty-acid synthase induces caspase-8-mediated tumor cell apoptosis by up-regulating DDIT4. Journal of Biological Chemistry 283: 31378-31384.

  145. Richardson AD, Yang C, Osterman A, Smith JW. (2008) Central carbon metabolism in the progression of mammary carcinoma. Breast Cancer Research and Treatment 110: 297-307.

  146. Yang C, Richardson AD, Osterman A, Smith JW. (2008) Profiling of Central Metabolism in Human Cancer Cells by Two-dimensional NMR, GC-MS Analysis, and Isotopomer Modeling. Metabolomics 4:13-29.

  147. Ying Zou, Xiaokui Guo, Mathieu Picardeau, Hai Xu, Guoping Zhao* (2007) A comprehensive survey on isoleucine biosynthesis pathways in seven epidemic Leptospira interrogans reference strains of China. FEMS Microbiology Letters 269 (1): 90–96.

  148. Jiang S., C. Li, W. Zhang, Y. Cai, Y. Yang, S. Yang, W. Jiang*. (2007) Directed evolution and structural analysis of N-carbamoyl-D-amino acid amidohydrolase provide insights on recombinant protein solubility in Escherichia coli. Biochemical Journal 402:429-437.

  149. Wang, Jingang; Zhang, Qing; He, Huang; Yuan, Zhongyi; Ding, Dafu; Yang, Sheng*; Jiang, Weihong. Increasing Synthetic Performance of Penicillin G Acylase from Bacillus megaterium by Site-directed Mutagenesis. Applied Microbiology and Biotechnology. 2007 Apr;74(5):1023-30. Epub 2006 Dec 21.

  150. Zhou Z et al. 2007. Differentiation of Populations of the Pinewood Nematode Bursaphelenchus xylophilus between and within Pine Forests. Phytopathology 97(3): 304-310.

  151. Yongzhen Tao, Yang Chen, Yongqiang Wu, Yanling He, and Zhihua Zhou. 2007. High hydrogen yield from a two-step process of dark- and photo-fermentation of sucrose. International Journal of Hydrogen Energy 32 (2): 200-206.

  152. Xu M., Zhu Y., Zhang R., Shen M., Jiang W., Zhao G., and Qin, Z. (2006) Characterization of the Genetic Components of Streptomyces lividans Linear Plasmid SLP2 for Replication in Circular and Linear Modes. Journal of Bacteriology.188: 6851-6857[*]

  153. Huabao Zheng, Xiaolan Wang, Jun Chen, Ke Zhu, Yuhua Zhao, Yunliu Yang, Sheng Yang*, Weihong Jiang, Expression, purification and immobilization of His-tagged D-amino acid oxidase of Trigonopsis variabilis in Pichia pastoris. Applied Microbiology and Biotechnology 2006, 70(6): 683-9[*]

  154. Xiao-yan Che, Biao Di, Guo-ping Zhao*, Ya-di Wang, Li-wen Qiu, Wei Hao, Ming Wang, Peng-zhe Qin, Yu-fei Liu, Kwok-hong Chan, Vincent C. C. Cheng, and Kwok-yung Yuen. (2006). A Patient with Asymptomatic Severe Acute Respiratory Syndrome (SARS) and Antigenemia from the 2003-2004 Community Outbreak of SARS in Guangzhou, China. CID 43: e1-e5.

  155. Hong-Liang YANG, Xu-Cheng JIANG, Xiang-Yan ZHANG, Wen-Jun LI, Bao-Yu HU, Guo-Ping ZHAO and Xiao-Kui GUO (2006). Thrombocytopenia in the experimental leptospirosis of guinea pig is not related to disseminated intravascular coagulation. BMC infectious diseases 6:19.

  156. Zhang R., Yang Y., Fang P., Jiang C., Xu L., Zhu Y., Shen M., Xia H., Zhao J., Chen T., and Qin. Z (2006) Diversity of telomere palindromic sequences and replication genes among Streptomyces linear plasmids. Applied and Environmental Microbiology. 72(9):5728-5733.

  157. Tianfan Cheng, Maolin Chen, Huabao Zheng, Jingang Wang, Sheng Yang*, Weihong Jiang. Expression and Purification of Penicillin G Acylase Enzymes from Four Different Micro-organisms, and a Comparative Evaluation of Their Synthesis/Hydrolysis Ratios for Cephalexin. Protein Expression and Purification 2006, 46, 107-13.

  158. Kawai M, Shoda-Kagaya E, Maehara T, Zhou ZH, et al. 2006 Genetic structure of pine sawyer Monochamus alternatus (Coleoptera : Cerambycidae) populations in northeast Asia: Consequences of the spread of pine wilt disease. Environmental Entomology 35 (2): 569-579.

  159. Shen M., Fang P., Xu D., Zhang Y., Cao W., Zhu Y., Zhao J. and Qin, Z. (2006) Replication and Inheritance of Nocardia Plasmid pC1. FEMS Microbiological Letter, 261:47-52.

  160. Wen-Tao PENG, Jin WANG, Ting WU, Jian-Qiang HUANG, Jui-Shen CHIAO, and Guo-Ping ZHAO* (2006) Bacterial Type I Glutamine Synthetase of the Rifamycin SV Producing Actinomycete, Amycolatopsis mediterranei U32, is the Only Enzyme Responsible for Glutamine Synthesis under Physiological Conditions. Acta Biochim Biophys Sin. 38: 821-830.

  161. Zhang W., Y. Liu, H. Zheng, S. Yang, W. Jiang*. Improving the activity and stability of GL-7-ACA acylase CA130 by site-directed mutagenesis. Appl. Environ. Microbiol. 2005, 71(9): 5290-5296. [*]

  162. Huai-Dong Song, Chang-Chun Tu, et al., Guo-Ping Zhao* (2005). Cross-host evolution of SARS coronavirus in palm civet and human. Proc. Natl. Acad. Sci. USA 102: 2430-2435[*]

  163. Wei Qian, …, Guo-Ping Zhao*, Ji-Liang Tang, Chaozu He (2005). Comparative and Functional Genomic Analyses of the Pathogenicity of Phytopathogen Xanthomonas campestris pv. campestris. Genome Res. 15: 757-767.

  164. Xue-Xia Miao, …, Guo-Ping Zhao*, Yong-Ping Huang (2005). Simple sequence repeat-based consensus linkage map of Bombyx mori. Proc. Natl. Acad. Sci. USA 102: 16305-16308.

  165. Xiu-Xia Qu, Pei Hao, Xi-Jun Song, Si-Ming Jiang, Yan-Xia Liu, Pei-Gang Wang, Xi Rao, Huai-Dong Song, Sheng-Yue Wang, Yu Zuo, Ai-Hu Zheng, Min Luo, Hua-Lin Wang, Fei Deng, Han-Zhong Wang, Zhi-Hong Hu, Ming-Xiao Ding, Guo-Ping Zhao*, and Hong-Kui Deng (2005). Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy. J. Biol. Chem. 280: 29588-29595.

  166. Huai-Dong Song, …, Guo-Ping Zhao* (2005). Cross-host evolution of SARS coronavirus in palm civet and human. Proc. Natl. Acad. Sci. USA 102: 2430-2435.

  167. Rossa W.K. Chiu, Stephen S.C. Chim, Yu-kwan Tong, Kitty S.C. Fung, Paul K.S. Chan, Guo-ping Zhao and Y.M. Dennis Lo (2005). Tracing SARS Coronavirus Variant with Large Genomic Deletion. Emerging Infectious Diseases 11: 168-170.

  168. Cheng A., W. Zhang, Y. Xie, W. Jiang, E. Arnold, S.G. Sarafianos, J. Ding. Expression, purification, and characterization of SARS coronavirus RNA polymerase. Virology. 2005, 335:165-176.

  169. Cai G., S. Zhu, X. Wang, W. Jiang*. Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from Achromobacter xylosoxidans. FEMS Microbiol. Lett. 2005, 249:15-21.

  170. Chen L., H. Yu, Y. Lu and W. Jiang. Cloning and preliminary characterization of lh3 gene encoding a putative acetyltransferase from a rifamycin sv-producing strain Amycolatopsis mediterranei. Biotechnology Letters 2005, 27(15):1129-1134.

  171. Changying Yu, Chunshan Gui, Haibin Luo, Lili Chen, Liang Zhang, Hao Yu, Sheng Yang, Weihong Jiang, Jianhua Shen, Xu Shen and Hualiang Jiang. Folding of SARS coronavirus spike glycoprotein immunological fragment (SARS_S1b): thermodynamic and kinetic investigation correlating with 3D structural modeling. Biochemistry. 2005, 44, 1453-1463.

  172. Zhao Yong, Li Wu, Zhou Zhihua, Wang Linghua, Pan Yingjie, Zhao Liping. 2005. Dynamics of microbial community structure and cellulolytic activity in agricultural soil amended with two biofertilizers. European Journal of Soil Biology 41: 21-29.

  173. Hai Xu, Yuzhen Zhang, Xiaokui Guo, Shuangxi Ren, Andreas A. Staempfli, Juishen Chiao, Weihong Jiang, and Guoping Zhao* (2004). Isoleucine biosynthesis in Leptospira interrogans serotype lai strain #56601 proceeds via a threonine-independent pathway. J Bacteriol 186: 5400-5409. [*]

  174. The Chinese SARS molecular epidemiology consortium (Guo-Ping Zhao* as correspondent of the whole work) (2004). Molecular Evolution of the SARS Coronavirus During the Course of the SARS Epidemic in China. Science 303: 1666-1669. [*]

  175. Shuang-Xi Ren, Gang Fu, Xiu-Gao Jiang, Rong Zeng, Guo-Ping Zhao* (2003). Unique Physiological and Pathogenic Features of Leptospira interrogans Revealed by Whole Genome Sequencing. Nature 422: 888-893. [*]

  176. Yue-Qing Zhang, Shuang-Xi Ren, Hua-Lin Li, Yong-Xiang Wang, Gang Fu, Jian Yang, Zhi-Qiang Qin, You-Gang Miao, Wen-Yi Wang, Run-Sheng Chen, Yan Shen, , Zhu Chen, Zheng-Hong Yuan, Guo-Ping Zhao, Di Qu, Antoine Danchin and Yu-Mei Wen (2003). Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain ATCC 12228. Mol Microbiol 49: 1577-1593.

  177. Ze-Guang Han, Guo-Ping Zhao and Zhu Chen (2003). Transcriptome study in China. C. R. Biologies 326: 949-957.

  178. Zhu S., Y. Yang, G. Zhao, W. Jiang (2003). A rapid and specific method to screen environmental microorganisms for cephalosporin acylase activity. J Microbiol Meth 54: 131-13.

  179. Ding X., Y. Tian, J. Chiao, G. Zhao, W. Jiang (2003). Stability of plasmid pA387 derivatives in Amycolatopsis mediterranei producing rifamycin. Biotechnol Lett 25: 1647-1652.

  180. Yu C, Meng X, Zhang S, Zhao G, Hu L, Kong X (2003). A 3-nucleotide deletion in the polypyrimidine tract of intron 7 of the DFNA5 gene causes nonsyndromic hearing impairment in a Chinese family. Genomics 82: 575-9.

  181. Xu Z., Y. Liu, Y. Yang, W. Jiang*, E. Arnold, J. Ding*. Crystal structure of D-hydantoinase from Burkholderia pickettii at a resolution of 2.7 Angstroms: insights into the molecular basis of enzyme thermostability. J. Bacteriol. 2003, 185: 4038-4049. [*]

  182. Zhou Z, Zhou L P, Chen M J, Zhang Y L, Li R B, Yang S, Yuan Z Y, Purification and characterization of Alcaligenes faecalis penicillin G acylase expressed in Bacillus subtilis. Acta Biochimica et Biophysica Sinica 2003, 35(5):416-22.

  183. Zhou Z, Zhang A H, Wang J R, Chen M L, Li R B, Yang S*, Yuan Z Y*. Improving the specific synthetic activity of a penicillin g acylase using DNA family shuffling. ACTA Biochimica et Biophysica Sinica 2003, 35(6):573-9.

  184. Zhou ZH, Miwa M, Nara K, et al. 2003 Patch establishment and development of a clonal plant, Polygonum cuspidatum, on Mount Fuji. Molecular Ecology 12 (6): 1361-1373.

  185. Kanchanaprayudh J, Zhou Z, Yomyart S, et al. 2003 A new species, Pisolithus abditus, an ectomycorrhizal fungus associated with dipterocarps in Thailand. Mycotaxon 88: 463-467.

  186. Nara K, Nakaya H, Wu BY, Zhou ZH, et al. 2003 Underground primary succession of ectomycorrhizal fungi in a volcanic desert on Mount Fuji. New Phytologist 159 (3): 743-756.

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Guidelines on Research Project Application
On the policy of the "Being open, fluidic and cooperative", The Key laboratary of Synthetic Biology (KLSB) warmly opens to the Chinese and foreign scientists. The Laboratory aims at the cutting-edge research in synthetic biology to address global challenges in areas related to energy, human health and the environment. The research subjects include characterization and design of functional biological parts, processing systems and organisms, production of biomaterial and energy molecules through modification and synthesis of biosystems as well as innovation of synthetic biology technology. Current research is going along the following three lines: 1. Assembly of large DNA fragments based on site-specific recombination system; 2. High-throughput DNA synthesis with micro-fluid chip.
The content of research projects for application must belong to the above areas, and with close relation to the current research projects. Chinese and foreign scientists with doctoral degrees or with a title equal or equivalent to or above assistant professor engaging in the study of synthetic biology may propose an application (The application from the Ph.D students in study is not accepted in principle). The applicants should have a certain advanced academic level, a sound research basis in their applied areas, and their research plans must be feasible.
Applicants may ask for an application form from KLSB, fill and print it with a computer. Applicants must send the copies of the application form (6 copies) with their curricula vitae and two recommendation letters before September 30 each year to KLSB. The computer version must be sent by express mail or through E-mail. After the first deliberation by specialists the applications will be carefully screened and selectedby the Academic Committee of KLSB. After getting the approval of the director and chairman of Academic Committee of KLSB the applicants are accepted as visiting scholars. Certain grant supports will be provided in accordance with the current level of funding.

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