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National Key Laboratory of Plant Molecular Genetics
Laboratory of Photosynthesis and Environmental Biology
Key Laboratory of Synthetic Biology
National Center for Gene Research, CAS
Research Center for Insect Sciences
Shanghai Entomological Museum, CAS
Key Laboratory of Synthetic Biology

The Key Laboratory of Synthetic Biology was established in Chinese Academy of Science in 2008 through reorganization of the Laboratory of Molecular Microbiology, The laboratory currently consists of 7 research groups including 12 principal investigators and about 70 graduate students. The director of laboratory is Prof. Guo-Ping Zhao and the academic committee director is Prof. Sheng-Li Yang.

The Laboratory aims at the cutting-edge research in synthetic biology to address global challenges in areas related to energy, human health and the environment. The research subjects include characterization and design of functional biological parts, processing systems and organisms, production of biomaterial and energy molecules through modification and synthesis of biosystems as well as innovation of synthetic biotechnology.

Research Groups:

Prof. Dr. Guo-Ping Zhao
Microbial Functional Genomics Group

Prof. Dr. Wei-Hong Jiang
Microbial Metabolic Regulation and Enzyme Engineering Group

Prof. Dr. Zhong-Jun Qin
Molecular Genetics of Actinomycetes and Genomic Engineering Group
　

Prof. Dr. Lai-Geng Li
Biomass and Bioenergy Group

Prof. Dr. Sheng Yang
Microbial Metabolic Regulation and Enzyme Engineering Group

Prof. Dr. Zhi-Hua Zhou
Microbial Metagenomics and Bioenergy Conversion Group
　

Prof. Dr. Chen Yang
Microbial Metabolic Engineering and Comparative Genomics Group

Prof. Dr. Peng Zhang
Cassava and Sweetpotato Biotechnology Group

 　

　

Academic Committee:
Sheng-Li Yang Academician Shanghai Research Center of Biotechnology, CAS
Zi-Xin Deng Academician Shanghai Jiao Tong University
Guo-Ping Zhao Academician Institute of Plant Physiology and Ecology, SIBS, CAS
Lu-Hua Lai Professor Peking University
Li Huang Professor Institute of Microbiology, CAS
Hai-Yan Liu Professor University of Science and Technology of China
Jia-Rui Wu Professor Shanghai Institutes of Biological Sciences, CAS
Si-Liang Zhang Professor East China University of Science and Technology
Dai-Jie Chen Professor Shanghai Institute of Pharmaceutical Industry
Lei Wang Professor Nankai University
Zhi-Hao Sun Professor Jiangnan University
Yi-Xue Li Professor Shanghai Center for Bioinformation Technology
Wen Liu Professor Shanghai Institute of Organic Chemistry, CAS
Wei-Hong Jiang Professor Institute of Plant Physiology and Ecology, SIBS, CAS
Hong-Wei Xue Professor Institute of Plant Physiology and Ecology, SIBS, CAS

Science Advisory Board:
Lu Yu Academician Institute of Theoretical Physics, CAS
Yun-Yu Shi Academician University of Science and Technology of China
Guo-Qiang Lin Academician Shanghai Institute of Organic Chemistry, CAS
Li-He Guo Professor Institute of Biochemistry and Cell Biology, SIBS, CAS
Zhang-Cheng Tang Professor Institute of Plant Physiology and Ecology, SIBS, CAS

Publications:

  1. Pinchuk, G.E., Rodionov, D.A., Yang, C., Li, X., Osterman, A., Dervyn, E., Geydebrekht, O.V., Reed, S.B., Romine, M.F., Collart, F.R., Scott, J.H., Fredrickson, J.K., Beliaev, A.S. (2009). Genomic reconstruction of Shewanella oneidensis MR-1 metabolism reveals a previously uncharacterized machinery for lactate utilization. Proc. Natl. Acad. Sci. USA. 106: 2874-2879.

  2. Yang J, Zhang Y, Xu J, Geng Y, Chen X, Yang H, Wang S, Wang H, Jiang X, Guo X, Zhao G. (2009) Serum activity of platelet-activating factor acetylhydrolase is a potential clinical marker for leptospirosis pulmonary hemorrhage. PloS one. 4(1):e4181.

  3. Xijun Ou, Bo Zhang, Lin Zhang, Guoping Zhao* and Xiaoming Ding* (2009) Characterization of rrdA, a TetR family protein gene involved in the regulation of secondary metabolism in Streptomyces coelicolor. Appl. Environ. Microbiol. 75(7): 2158-2165.

  4. Cai Y., P, Trodler, S. Jiang, W. Zhang, Y. Wu, Y. Lu, S. Yang, W. Jiang. (2009) Isolation and molecular characterization of a novel d-hydantoinase from Jannaschia sp. CCS1. FEBS Journal DOI: 10.1111/j.1742-4658.2009.07077.X

  5. J Yu., C. Xu. F. Dong, Y. Yang., W. Jiang*, S.Yang* (2009) Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metabolic Engineering. DOI: 10.1016/j.ymben.2009.06.002.

  6. Xie P., Zeng A., and Qin Z (2009) cmdABCDEF, a cluster of genes encoding membrane proteins for differentiation and antibiotic production in Streptoymyces coelicolor A3(2). BMC Microbiology. 9:157.

  7. Junjie YANG, Weihong JIANG, Sheng YANG* (2009). mazF as a counter-selectable marker for unmarked genetic modification of Pichia pastoris. FEMS Yeast Res. 9(4):600-609.

  8. Yang Gu, Sheng YANG*, Weihong JIANG*.(2009) Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coli. Journal of Biotechnology, accepted

  9. Liu F.H, S.B. Wang, J.S. Zhang, J. Zhang, X. Yan, H.K. Zhou, G.P. Zhao and Z.H. Zhou. (2009) The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis. Journal of Applied Microbiology 106:952-966.

  10. Liu F.H, G.H. Lin, G. Gao, B.Q. Qin, J.S. Zhang, G.P. Zhao, Z.H. Zhou and J.H. Shen. (2009) Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis (DGGE). Journal of Applied Microbiology 106:1022-1032.

  11. Hong YU, Jian LI, Dalong ZHANG, Yunliu YANG, Weihong JIANG, Sheng YANG* (2009). Improving the thermostability of N-carbamyl-D-amino acid amidohydrolase by error-prone PCR. Appl. Microbiol. Biotechnol. 82(2) 279.

  12. Zhang R, Xia H, Guo P. and Qin Z (2009) Variation in the replication loci of Streptomyces linear plasmids. FEMS Microbiological Letter. 290:209-216.

  13. Wang W., D. Shu, L. Chen, W. Jiang, Y. Lu. (2009) Cross-talk between an orphan response regulator and a noncognate histidine kinase in Streptomyces coelicolor. FEMS Microbiol Lett 294:150-156.

  14. Fan XY, Ma H, Guo J, Li ZM, Cheng ZH, Guo SQ, Zhao GP* (2009) A novel differential expression system for gene modulation in Mycobacteria. Plasmid. 61(1): 39-46. Epub 2008 Oct 25.

  15. Tian Y., Jiang W., Zhao G., and Qin Z (2009) In vivo conjugation-coupled recombinational cloning of a Streptomyces lividans chromosomal telomeric DNA using a linear plasmid. Biotechnology Letter. 31(8):1253-1258.

  16. Shu D., L. Chen, W. Wang, Z. Yu, C. Ren, W. Zhang, S. Yang, Y. Lu*, W. Jiang*. (2009) afsQ1-Q2-sigQ is a pleiotropic but conditionally required signal transduction system for both secondary metabolism and morphological development in Streptomyces coelicolor. Applied Microbiology and Biotechnology 81:1149-1160.

  17. Yu H., J. Li, D. Zhang, Y. Yang, W. Jiang, S.Yang*. (2009) Improving the thermostability of N-Carbamyl-D-amino acid amidohydrolase by error-prone PCR. Applied Microbiology and Biotechnology 82:279-285.

  18. Gu Y., S. Hu, J. Chen, L. Shao , H. He , Y. Yang , S. Yang*, W. Jiang*.(2009) Ammonium acetate enhances solvent production by Clostridium acetobutylicum EA 2018 using cassava as a fermentation medium. Journal of Industrial Microbiology and Biotechnology. DOI: 10.1007/s10295-009-0604-1.

  19. Hu S., J. Chen, Z.Yang, L. Shao, H. Bai, J. Luo,W. Jiang, Y. Yang* .(2009) Coupled bioconversion for preparation of N-acetyl-Dneuraminic acid using immobilized N-acetyl-D-glucosamine-2-epimerase and N-acetyl-D-neuraminic acid lyase, Applied Microbiology and Biotechnology. DOI 10.1007/s00253-009-2163-9.

  20. 刘桂玲, 郑建利, 柳新明, 陈宝芳, 张鹏. (2009) 不同基因型甘薯品种产量与地上部农艺性状及主要气象因子关联度分析. 中国农学通报. 25(05):125-131.

  21. 胡世元, 杨蕴刘, 杨晟, 姜卫红. (2009) 丙酮丁醇梭菌的特性及遗传改造. 工业生物技术发展报告. 科学出版社 94-103.

  22. Chen L, Lu Y, Chen J, Zhang W, Shu D, Qin Z, Yang, S and Jiang W. (2008) Characterization of a negative regulator AveI for avermectin biosynthesis in Streptomyces avermitilis NRRL8165. Applied Microbiolology and Biotechnology 80:277-286.

  23. Min Li, Baohong Wang, Menghui Zhang, Mattias Rantalainen, Shengyue Wang, Haokui Zhou, Yan Zhang, Jian Shen, Xiaoyan Pang, Meiling Zhang, Hua Wei, Yu Chen†, Haifeng Lu, Jian Zuo, Mingming Su, Yunping Qiu, Wei Jia, Chaoni Xiao, Leon M. Smith, Shengli Yang, Elaine Holmes, Huiru Tang, Guoping Zhao*, Jeremy K. Nicholson, Lanjuan Li, and Liping Zhao (2008) Symbiotic gut microbes modulate human metabolic phenotypes. Proc. Natl. Acad. Sci. USA. 105: 2117-2122.

  24. Jun MA, Peng ZHANG, Zilong ZHANG, Manwu ZHA, Hai XU, Guoping ZHAO* and Jianping DING (2008) Molecular basis of the substrate specificity and the catalytic mechanism of citramalate synthase from Leptospira interrogans. Biochem. J. 415, 45-56.

  25. Xiaonan Yang, Hongliang Yang, Gangqiao Zhou, and Guo-Ping Zhao* (2008) Infectious Disease in the Genomic Era. Annu. Rev. Genomics Hum. Genet. 9:21-48.

  26. Lin Zhang, Xijun Ou, Guoping Zhao*, and Xiaoming Ding* (2008) Highly efficient in vitro site-specific recombination system based on Streptomyces phage BT1 integrase. J. Bacteriol. 190: 6392-6397.

  27. Xijun Ou, Bo Zhang, Lin Zhang, Kai Dong, Chun Liu, Guoping Zhao*, and Xiaoming Ding* (2008) SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145. Acta Biochim Biophys Sin 40: 877-882.

  28. Qin JH, Zhang Q, Zhang ZM, Zhong Y, Yang Y, Hu BY, Zhao GP, Guo XK. (2008) Identification of a novel prophage-like gene cluster actively expressed in both virulent and avirulent strains of Leptospira interrogans serovar lai. Infect Immun. 76(6): 2411-9.

  29. Zhang YX, Geng Y, Yang JW, Guo XK, Zhao GP. (2008) Cytotoxic activity and probable apoptotic effect of Sph2, a sphigomyelinase hemolysin from Leptospira interrogans strain Lai. BMB Rep. 41(2): 119-25.

  30. Zhang R., Zeng A., Fang P., and Qin Z (2008) Characterization of the replication loci of Streptomyces circular plasmids pFP11 and pFP1 and their ability to propagate in linear mode with artificially attached telomeres. Applied and Environmental Microbiology. 74(11):3368-3376.

  31. Zhao J., Zhong L., Shen M., Xia Z., Cheng Q., Sun X., Zhao G., Li Y., and Qin Z (2008) Discovery of an autonomously-replicating plasmid pMF1 from Myxococcus fulvus and the development of a gene cloning system in Myxococcus Xanthus. Applied and Environmental Microbiology. 74(7):1980-1987.

  32. Tao Y, et al. and Zhou Z 2008 Characteristics of a new photosynthetic bacterial strain for hydrogen production and its application in wastewater treatment. International Journal of Hydrogen Energy 33: 963-973.

  33. Yang C, Rodionov DA, Rodionova IA, Li X, Osterman A. (2008) Glycerate 2-kinase family and genomic reconstruction of related metabolic pathways. Case study in Thermotoga maritima. Journal of Bacteriology 190: 1773-1782.

  34. Knowles LM, Yang C, Osterman A, Smith JW. (2008) Inhibition of fatty-acid synthase induces caspase-8-mediated tumor cell apoptosis by up-regulating DDIT4. Journal of Biological Chemistry 283: 31378-31384.

  35. Richardson AD, Yang C, Osterman A, Smith JW. (2008) Central carbon metabolism in the progression of mammary carcinoma. Breast Cancer Research and Treatment 110: 297-307.

  36. Yang C, Richardson AD, Osterman A, Smith JW. (2008) Profiling of Central Metabolism in Human Cancer Cells by Two-dimensional NMR, GC-MS Analysis, and Isotopomer Modeling. Metabolomics 4:13-29.

  37. Ying Zou, Xiaokui Guo, Mathieu Picardeau, Hai Xu, Guoping Zhao* (2007) A comprehensive survey on isoleucine biosynthesis pathways in seven epidemic Leptospira interrogans reference strains of China. FEMS Microbiology Letters 269 (1): 90–96.

  38. Jiang S., C. Li, W. Zhang, Y. Cai, Y. Yang, S. Yang, W. Jiang*. (2007) Directed evolution and structural analysis of N-carbamoyl-D-amino acid amidohydrolase provide insights on recombinant protein solubility in Escherichia coli. Biochemical Journal 402:429-437.

  39. Wang, Jingang; Zhang, Qing; He, Huang; Yuan, Zhongyi; Ding, Dafu; Yang, Sheng*; Jiang, Weihong. Increasing Synthetic Performance of Penicillin G Acylase from Bacillus megaterium by Site-directed Mutagenesis. Applied Microbiology and Biotechnology. 2007 Apr;74(5):1023-30. Epub 2006 Dec 21.

  40. Zhou Z et al. 2007. Differentiation of Populations of the Pinewood Nematode Bursaphelenchus xylophilus between and within Pine Forests. Phytopathology 97(3): 304-310.

  41. Yongzhen Tao, Yang Chen, Yongqiang Wu, Yanling He, and Zhihua Zhou. 2007. High hydrogen yield from a two-step process of dark- and photo-fermentation of sucrose. International Journal of Hydrogen Energy 32 (2): 200-206.

  42. Xu M., Zhu Y., Zhang R., Shen M., Jiang W., Zhao G., and Qin, Z. (2006) Characterization of the Genetic Components of Streptomyces lividans Linear Plasmid SLP2 for Replication in Circular and Linear Modes. Journal of Bacteriology.188: 6851-6857[*]

  43. Huabao Zheng, Xiaolan Wang, Jun Chen, Ke Zhu, Yuhua Zhao, Yunliu Yang, Sheng Yang*, Weihong Jiang, Expression, purification and immobilization of His-tagged D-amino acid oxidase of Trigonopsis variabilis in Pichia pastoris. Applied Microbiology and Biotechnology 2006, 70(6): 683-9[*]

  44. Xiao-yan Che, Biao Di, Guo-ping Zhao*, Ya-di Wang, Li-wen Qiu, Wei Hao, Ming Wang, Peng-zhe Qin, Yu-fei Liu, Kwok-hong Chan, Vincent C. C. Cheng, and Kwok-yung Yuen. (2006). A Patient with Asymptomatic Severe Acute Respiratory Syndrome (SARS) and Antigenemia from the 2003-2004 Community Outbreak of SARS in Guangzhou, China. CID 43: e1-e5.

  45. Hong-Liang YANG, Xu-Cheng JIANG, Xiang-Yan ZHANG, Wen-Jun LI, Bao-Yu HU, Guo-Ping ZHAO and Xiao-Kui GUO (2006). Thrombocytopenia in the experimental leptospirosis of guinea pig is not related to disseminated intravascular coagulation. BMC infectious diseases 6:19.

  46. Zhang R., Yang Y., Fang P., Jiang C., Xu L., Zhu Y., Shen M., Xia H., Zhao J., Chen T., and Qin. Z (2006) Diversity of telomere palindromic sequences and replication genes among Streptomyces linear plasmids. Applied and Environmental Microbiology. 72(9):5728-5733.

  47. Tianfan Cheng, Maolin Chen, Huabao Zheng, Jingang Wang, Sheng Yang*, Weihong Jiang. Expression and Purification of Penicillin G Acylase Enzymes from Four Different Micro-organisms, and a Comparative Evaluation of Their Synthesis/Hydrolysis Ratios for Cephalexin. Protein Expression and Purification 2006, 46, 107-13.

  48. Kawai M, Shoda-Kagaya E, Maehara T, Zhou ZH, et al. 2006 Genetic structure of pine sawyer Monochamus alternatus (Coleoptera : Cerambycidae) populations in northeast Asia: Consequences of the spread of pine wilt disease. Environmental Entomology 35 (2): 569-579.

  49. Shen M., Fang P., Xu D., Zhang Y., Cao W., Zhu Y., Zhao J. and Qin, Z. (2006) Replication and Inheritance of Nocardia Plasmid pC1. FEMS Microbiological Letter, 261:47-52.

  50. Wen-Tao PENG, Jin WANG, Ting WU, Jian-Qiang HUANG, Jui-Shen CHIAO, and Guo-Ping ZHAO* (2006) Bacterial Type I Glutamine Synthetase of the Rifamycin SV Producing Actinomycete, Amycolatopsis mediterranei U32, is the Only Enzyme Responsible for Glutamine Synthesis under Physiological Conditions. Acta Biochim Biophys Sin. 38: 821-830.

  51. Zhang W., Y. Liu, H. Zheng, S. Yang, W. Jiang*. Improving the activity and stability of GL-7-ACA acylase CA130 by site-directed mutagenesis. Appl. Environ. Microbiol. 2005, 71(9): 5290-5296. [*]

  52. Huai-Dong Song, Chang-Chun Tu, et al., Guo-Ping Zhao* (2005). Cross-host evolution of SARS coronavirus in palm civet and human. Proc. Natl. Acad. Sci. USA 102: 2430-2435[*]

  53. Wei Qian, …, Guo-Ping Zhao*, Ji-Liang Tang, Chaozu He (2005). Comparative and Functional Genomic Analyses of the Pathogenicity of Phytopathogen Xanthomonas campestris pv. campestris. Genome Res. 15: 757-767.

  54. Xue-Xia Miao, …, Guo-Ping Zhao*, Yong-Ping Huang (2005). Simple sequence repeat-based consensus linkage map of Bombyx mori. Proc. Natl. Acad. Sci. USA 102: 16305-16308.

  55. Xiu-Xia Qu, Pei Hao, Xi-Jun Song, Si-Ming Jiang, Yan-Xia Liu, Pei-Gang Wang, Xi Rao, Huai-Dong Song, Sheng-Yue Wang, Yu Zuo, Ai-Hu Zheng, Min Luo, Hua-Lin Wang, Fei Deng, Han-Zhong Wang, Zhi-Hong Hu, Ming-Xiao Ding, Guo-Ping Zhao*, and Hong-Kui Deng (2005). Identification of two critical amino acid residues of the severe acute respiratory syndrome coronavirus spike protein for its variation in zoonotic tropism transition via a double substitution strategy. J. Biol. Chem. 280: 29588-29595.

  56. Huai-Dong Song, …, Guo-Ping Zhao* (2005). Cross-host evolution of SARS coronavirus in palm civet and human. Proc. Natl. Acad. Sci. USA 102: 2430-2435.

  57. Rossa W.K. Chiu, Stephen S.C. Chim, Yu-kwan Tong, Kitty S.C. Fung, Paul K.S. Chan, Guo-ping Zhao and Y.M. Dennis Lo (2005). Tracing SARS Coronavirus Variant with Large Genomic Deletion. Emerging Infectious Diseases 11: 168-170.

  58. Cheng A., W. Zhang, Y. Xie, W. Jiang, E. Arnold, S.G. Sarafianos, J. Ding. Expression, purification, and characterization of SARS coronavirus RNA polymerase. Virology. 2005, 335:165-176.

  59. Cai G., S. Zhu, X. Wang, W. Jiang*. Cloning, sequence analysis and expression of the gene encoding a novel wide-spectrum amidase belonging to the amidase signature superfamily from Achromobacter xylosoxidans. FEMS Microbiol. Lett. 2005, 249:15-21.

  60. Chen L., H. Yu, Y. Lu and W. Jiang. Cloning and preliminary characterization of lh3 gene encoding a putative acetyltransferase from a rifamycin sv-producing strain Amycolatopsis mediterranei. Biotechnology Letters 2005, 27(15):1129-1134.

  61. Changying Yu, Chunshan Gui, Haibin Luo, Lili Chen, Liang Zhang, Hao Yu, Sheng Yang, Weihong Jiang, Jianhua Shen, Xu Shen and Hualiang Jiang. Folding of SARS coronavirus spike glycoprotein immunological fragment (SARS_S1b): thermodynamic and kinetic investigation correlating with 3D structural modeling. Biochemistry. 2005, 44, 1453-1463.

  62. Zhao Yong, Li Wu, Zhou Zhihua, Wang Linghua, Pan Yingjie, Zhao Liping. 2005. Dynamics of microbial community structure and cellulolytic activity in agricultural soil amended with two biofertilizers. European Journal of Soil Biology 41: 21-29.

  63. Hai Xu, Yuzhen Zhang, Xiaokui Guo, Shuangxi Ren, Andreas A. Staempfli, Juishen Chiao, Weihong Jiang, and Guoping Zhao* (2004). Isoleucine biosynthesis in Leptospira interrogans serotype lai strain #56601 proceeds via a threonine-independent pathway. J Bacteriol 186: 5400-5409. [*]

  64. The Chinese SARS molecular epidemiology consortium (Guo-Ping Zhao* as correspondent of the whole work) (2004). Molecular Evolution of the SARS Coronavirus During the Course of the SARS Epidemic in China. Science 303: 1666-1669. [*]

  65. Shuang-Xi Ren, Gang Fu, Xiu-Gao Jiang, Rong Zeng, Guo-Ping Zhao* (2003). Unique Physiological and Pathogenic Features of Leptospira interrogans Revealed by Whole Genome Sequencing. Nature 422: 888-893. [*]

  66. Yue-Qing Zhang, Shuang-Xi Ren, Hua-Lin Li, Yong-Xiang Wang, Gang Fu, Jian Yang, Zhi-Qiang Qin, You-Gang Miao, Wen-Yi Wang, Run-Sheng Chen, Yan Shen, , Zhu Chen, Zheng-Hong Yuan, Guo-Ping Zhao, Di Qu, Antoine Danchin and Yu-Mei Wen (2003). Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain ATCC 12228. Mol Microbiol 49: 1577-1593.

  67. Ze-Guang Han, Guo-Ping Zhao and Zhu Chen (2003). Transcriptome study in China. C. R. Biologies 326: 949-957.

  68. Zhu S., Y. Yang, G. Zhao, W. Jiang (2003). A rapid and specific method to screen environmental microorganisms for cephalosporin acylase activity. J Microbiol Meth 54: 131-13.

  69. Ding X., Y. Tian, J. Chiao, G. Zhao, W. Jiang (2003). Stability of plasmid pA387 derivatives in Amycolatopsis mediterranei producing rifamycin. Biotechnol Lett 25: 1647-1652.

  70. Yu C, Meng X, Zhang S, Zhao G, Hu L, Kong X (2003). A 3-nucleotide deletion in the polypyrimidine tract of intron 7 of the DFNA5 gene causes nonsyndromic hearing impairment in a Chinese family. Genomics 82: 575-9.

  71. Xu Z., Y. Liu, Y. Yang, W. Jiang*, E. Arnold, J. Ding*. Crystal structure of D-hydantoinase from Burkholderia pickettii at a resolution of 2.7 Angstroms: insights into the molecular basis of enzyme thermostability. J. Bacteriol. 2003, 185: 4038-4049. [*]

  72. Zhou Z, Zhou L P, Chen M J, Zhang Y L, Li R B, Yang S, Yuan Z Y, Purification and characterization of Alcaligenes faecalis penicillin G acylase expressed in Bacillus subtilis. Acta Biochimica et Biophysica Sinica 2003, 35(5):416-22.

  73. Zhou Z, Zhang A H, Wang J R, Chen M L, Li R B, Yang S*, Yuan Z Y*. Improving the specific synthetic activity of a penicillin g acylase using DNA family shuffling. ACTA Biochimica et Biophysica Sinica 2003, 35(6):573-9.

  74. Zhou ZH, Miwa M, Nara K, et al. 2003 Patch establishment and development of a clonal plant, Polygonum cuspidatum, on Mount Fuji. Molecular Ecology 12 (6): 1361-1373.

  75. Kanchanaprayudh J, Zhou Z, Yomyart S, et al. 2003 A new species, Pisolithus abditus, an ectomycorrhizal fungus associated with dipterocarps in Thailand. Mycotaxon 88: 463-467.

  76. Nara K, Nakaya H, Wu BY, Zhou ZH, et al. 2003 Underground primary succession of ectomycorrhizal fungi in a volcanic desert on Mount Fuji. New Phytologist 159 (3): 743-756.

  77. Lian CL, Oishi R, Miyashita N, Zhou ZH, et al. 2003 Genetic structure and reproduction dynamics of Salix reinii during primary succession on Mount Fuji, as revealed by nuclear and chloroplast microsatellite analysis. Molecular Ecology 12 (3): 609-618.

  78. Qin, Z., Shen, M. and Cohen, S. N. (2003) Identification and characterization of a pSLA2 plasmids locus required for linear DNA replication and circular plasmids stable inheritance in Streptomyces lividans. Journal of Bacteriology, 185 (22),6575-6582.

  79. Zhou Z, Zhang A H, Wang J R, Chen M L, Li R B, Yang S*, Yuan Z Y*. Improving the specific synthetic activity of a penicillin G acylase using DNA family shuffling. Acta biochimica et biophysica sinica 2003, 35(6):573-9.

  80. Huang X., R. Zeng, X. Ding, X. Mao, Y. Ding, Z. Rao, Y. Xie, W. Jiang*, G. Zhao*. Affinity alkylation of the Trp-B4 residue of the ß-subunit of the glutaryl 7-amidocephalosporanic acid acylase of Pseudomonas sp.130. J. Biol. Chem. 2002, 277(12):10256-10264. [*]

  81. Wang W., W. Zhang, H. Chen, J. Chiao, G. Zhao, W. Jiang*. Molecular and biochemical characterization of a novel two-component signal transduction system amrA-amkA involved in rifamycin SV production in Amycolatopsis mediterranei U32. Arch. Microbiol. 2002, 178: 376-386.

  82. Wang W., W. Zhang, J. Lu, Y. Yang, J. Chiao, G. Zhao, W. Jiang. MoeA, an enzyme in the molybdopterin synthesis pathway, is required for rifamycin SV production in Amycolatopsis mediterranei U32. Appl. Microbiol. & Biotechnol. 2002, 60: 139-146.

  83. Wang W., W. Zhang, H. Chen, J. Chiao, G. Zhao, W. Jiang. Molecular and biochemical characterization of a novel two-component signal transduction system amrA-amkA involved in rifamycin SV production in Amycolatopsis mediterranei U32. Arch. Microbiol. 2002, 178: 376-386.

  84. Yao Y., W. Zhang, R. Jiao, G. Zhao, W. Jiang. Efficient isolation of total RNA from antibiotic-producing bacterium Amycolatopsis mediterranei. J. Microbiol. Meth. 2002, 51(2):191-195.

  85. Gao J., W. Jiang, G. Zhao, J. Chiao. Cloning, sequencing and expression of a recA gene from Amycolatopsis mediterranei. Biotechnol. Lett. 2002, 24: 909-913.

  86. Zhou ZH, Hogetsu T. 2002 Subterranean community structure of ectomycorrhizal fungi under Suillus grevillei sporocarps in a Larix kaempferi forest. New Phytologist 154 (2): 529-539.

Guidelines on Research Project Application
On the policy of the "Being open, fluidic and cooperative", The Key laboratary of Synthetic Biology (KLSB) warmly opens to the Chinese and foreign scientists. The Laboratory aims at the cutting-edge research in synthetic biology to address global challenges in areas related to energy, human health and the environment. The research subjects include characterization and design of functional biological parts, processing systems and organisms, production of biomaterial and energy molecules through modification and synthesis of biosystems as well as innovation of synthetic biology technology. Current research is going along the following three lines: 1. Assembly of large DNA fragments based on site-specific recombination system; 2. High-throughput DNA synthesis with micro-fluid chip.
The content of research projects for application must belong to the above areas, and with close relation to the current research projects. Chinese and foreign scientists with doctoral degrees or with a title equal or equivalent to or above assistant professor engaging in the study of synthetic biology may propose an application (The application from the Ph.D students in study is not accepted in principle). The applicants should have a certain advanced academic level, a sound research basis in their applied areas, and their research plans must be feasible.
Applicants may ask for an application form from KLSB, fill and print it with a computer. Applicants must send the copies of the application form (6 copies) with their curricula vitae and two recommendation letters before September 30 each year to KLSB. The computer version must be sent by express mail or through E-mail. After the first deliberation by specialists the applications will be carefully screened and selectedby the Academic Committee of KLSB. After getting the approval of the director and chairman of Academic Committee of KLSB the applicants are accepted as visiting scholars. Certain grant supports will be provided in accordance with the current level of funding.

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