Our research interests fall into two main complementary areas (1) structure and physiological function of NAD(P)H complex (NDH) bound in thylakoid membranes; (2) interactions among proteins in the photosynthetic electron transfer chain.

Principal Investigator: Dr. Hua-Ling Mi, Professor. Email: mihl@sippe.ac.cn
Staff: Dr. Zi-Ying Gong, Dr. Min Xu, Research Assistants; Fen-Fei Wang, Research Intern
Graduate Students: Yan-Xia Wu; Peng Hu; Nan Shi; Meng-Meng Kong; Zhi-Hui He; Chun-Guang Chen; Jia-Fei Li
Graduated Students: Jia-Li Wei, 2002 (PhD); Min Xu, 2004 (PhD)
Visiting Scholar: Dr. Teruo Ogawa, Professor (Nagoya University, Japan)

Identification and Localization of the CupB Protein Involved in Constitutive CO2 Uptake in the Cyanobacterium, Synechocystis sp. Strain PCC 6803
Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane fraction. The protein was not detected in the membranes of the DndhD4 and DndhF4 mutants in which CupB was tagged with HM. We concluded that a CupB complex containing NdhD4 and NdhF4 is largely, if not exclusively, confined to the thylakoid membrane. Both CupB and NdhH were detected in a fraction containing protein complexes of 450 kDa, obtained after nickel column and gel filtration chromatography of the membranes solubilized with n-dodecyl-b-maltoside.

Major Publications:

1. Xu M, Bernát G, Singh A, Mi HL, Rögner M, Pakrasi HB, Ogawa T. (2008) Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems. Plant Cell Physiol. 49(11):1672-1677. doi:10.1093/pcp/pcn139.

2. Xu M, Ogawa T, Pakrasi HB, Mi HL*. (2008) Identification and localization of the CupB protein involved in constitutive CO2 uptake in the cyanobacterium, Synechocystis sp. strain PCC 6803. Plant Cell Physiol. 49: 994-997.

3. Ma WM, Mi HL*. (2008) Effect of exogenous glucose on the expression and activity of NADPH dehydrogenase complexes in the cyanobacterium Synechocystis sp. strain PCC 6803. Plant Physiology and Biochemistry 46: 775-779.

4. Ma WM, Deng Y, Mi HL*. (2008) Redox of Plastoquinone Pool Regulates Expression and Activity of NADPH Dehydrogenase Super-complex in Synechocystis sp. Strain PCC 6803. Current Microbiology 56: 189-193.

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