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 The cell nucleus, which contains the bulk of the genetic and epigenetic information of the cell, is an important and complex organelle. Recent progress in live-cell imaging opens a door towards understanding how the various subnuclear structures contribute to precise functioning of the genome and epigenome in living cells. We aim to identify and characterize essential plant subnuclear domains through advanced live-cell deconvolution or confocal microscopy in conjunction with genetic, molecular and biochemical approaches. Our studies contribute to how gene expression machinery is spatio-temporally coordinated in cell nuclei of a living organism.
Education Background & Academic Experience
Education:
August, 1985: BS, Department of Agronomy, Wannan Agricultural College (University of Science and Technology of China), Hefei, P.R. China
August, 1988: MS Class, Department of Agronomy, Nanjing Agricultural University, Nanjing, P.R. China
June, 1999: PhD, Department of Agronomy, Nanjing Agricultural University, Nanjing, P.R. China
Academic experience:
September, 1988: Teaching assistant, Department of Biology, Huangshan College, Huangshan, P.R. China
September, 1992: Lecture, Department of Biology, Huangshan College, Huangshan, P.R. China
July, 1999: Post-doctor, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany
December, 2001: Post-doctor, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
January, 2009: Principal investigator, Professor, Laboratory of Cell Biology of Plant Genome and Epigenome, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, P.R. China
Major Publications:
Fang Y, and Spector DL; Live cell Imaging of Plants. In: Jason Swedlow, Robert D. Goldman, and David L. Spector (Eds.). Live-cell Imaging: A Laboratory Manual (2th edition) Cold Spring Harbor Laboratory Press. 2009 Chapter 24 (in press). Rosin, F.M., Watanabe, N., Cacas, J.L., Kato, N., Arroyo, L.M., Fang Y, May, B., Vaughn,M., Simorowski, J., Ramu, U., McCombie, R.W., Spector DL, Martienssen, R.A., and Lam,E. (2008). Genome-wide tagging reveals location-dependent effects on transcription and chromatin organization in Arabidopsis. Plant Journal 55(3):514-25. Fang Y and Spector DL (2007). Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants. Current Biology 17(9):818-823. Fang Y and Spector DL (2005). Centromere positioning and dynamics in living Arabidopsis plants. Molecular Biology of the Cell 16(12):5710-5718. May BP, Lippman ZB, Fang Y, Spector DL, and Martienssen RA (2005). Differential regulation of strand-specific transcripts from Arabidopsis centromeric satellite repeats. PLoS Genetics 1(6):e79. Ono T, Fang Y, Spector DL, and Hirano T (2004). Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells. Molecular Biology of the Cell 15(7):3296-3308. Fang Y, Hearn S, and Spector DL (2004). Tissue-specific expression and dynamic organization of SR splicing factors in Arabidopsis. Molecular Biology of the Cell 15(6):2664-2673. Fang Y, Akula C, and Altpeter F. (2002). Agrobacterium-mediated barley (Hordeum vulgare L.) transformation using green fluorescent protein as a vital marker and sequence analysis of the T-DNA::genomic DNA junctions. Journal of Plant Physiology 159:1131-1138.
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